Biopolymers and cell. 1999; 15 (4): 297 - 299

Deckase of glucose level in vitro and in vivo by recombinant vector containing human preproinsulin gene

F. Ajamian, E. M. Suhorada, T. A. Ruban, T. A. Titok

The expression of human preproinsulin gene was studied. The fragment of genomic DNA, containing the sequences of pre¬proinsulin gene without its personal promoter was cloned in the vector plasmid DNA pTR-UF under control of cytomegalovirus promoter. The obtained recombinant plasmid DNA was tested in vitro, in Hep-2 of tissue culture and in vivo at streptozotocin diabetic mice by introducing the preparation into the liver. As a result of our experiment we found out the reduction of glucose level both in the cells of tissue culture and in the blood of the experimental animals. The presented results, in the author's opi¬nion, evidence about the availability of the expression of the human preproinsulin gene.