Biopolymers and cell. 1999; 15 (4): 283 - 288

Hybridomas producing the monoclonal antibodies to the cotton membrane proteins

Z. S. Khashimova, Ju. S. Mangutova, M. E. Suslo, V. B. Leontjev

Monoclonal antibodies (mcAB) to membrane antigens were gene¬rated by immunization of BALB/c mices with total plasma mem¬brane proteins from cotton seedlings. 12 stable hybridoma clones secreting mcAB directed to the components of the total membrane proteins were selected; reactivity of these mcAB was determined using ELJSA methods. McAB to surface epitopes of isolated cotton protoplasts, membrane and membrane-bound proteins, cell-wall protein (extensin), some groups of lectin-like proteins from cotton seeds have been described, their immunochemical cross-reactivity was discussed. McAB to cotton plasma-membrane proteins can be used as biochemical markers and for fractionation and membrane proteins functional activity study.