Biopolymers and cell. 1999; 15 (3): 247 - 251

Interaction of cyanine dyes with nucleic acids. 6,, Synthesis and spectroscopic properties of thiazole orange—amino acids conjugates

Sergiy M. Yarmoluk, Dmytro V. Kryvorotenko, Yuriy S. Gerasymchuk

The synthesis of amino acid (L-Trp and L-Tyr) derivatives of thiazole orange, monomethirte cyanine dye which significantly increases fluorescence intensity when bound to nucleic acids is described. Hydroxysiiccinimsde ester of Cyan 6 (2-[4-N-Carboxyethyl-quinoline)-methyl]-3-niethylberizihiazole-l,4-p-toluenesulfonatt) was used for the conjugation of dye with amino acids, interaction of obtained conjugates with nucleic acids was investigated using spectroscopic methods.