Biopolymers and cell. 1999; 15 (1): 28 - 37

The recombinant DNA pGins that contains expressing human preproinsulin gene has no mutagenic activity

L. L. Lukash, S. V. Podolskaya, A. A. Evseeenko, He. M. Suhorada, L. N. Neborachko, 7. S. Varsanova, T. P. Kociiubei

Uniike recombinant plasmids pBK322ins u pAins the plasmid DNA pGins at the concentration of 10 ng/rnl does not manifest mutagenic activity in. somatic mammalian cells. The at>sence of pGins mu-tagnnic activity is confirmed in different test-systems in vitro and in vivo and presumably is determined by the increasing of the expression and the functions of the preproinsulin gene under the action, of virus regulation elements. It is shown that the preliminary treatment of somatic mammalian cells by insulin preparation protects them against the mutagenic action of recombinant plasmid pfff)320, containing the genome of virus hepatitis B. The data obtained here testify to the participation of the expressing gene coding for insulin in the maintenance of genetic stability or cellular populations variation.