Biopolymers and cell. 1998; 14 (6): 544 - 548

The influence of granulocyte colony-stimulating factor «Granocyte» on active oxygen forms generation by neutrophils in vitro

E. I. Kovalenko, G. N. Semeiikova, E. N. Smirnova, S. N. Cherenkevich, Y. A. Razumovich, V. Gerein

Granulocyte colony-stimulating factors arc used at the therapy of myelosupressive conditions. The influence of granulocyte colony-stimulating factor «Granocyte» in vitro on active oxygen forms generation by neutrophils of healthy donors and myeloperoxidase activity of these cells was investigated at the adhesion of cells on tlif glass surface and the action of latex, phyfohemagglutinin and arachidonic acid. Researches were curried out by chemiluminescenl method with use of luminol. It was shown, that «Granocytc» increases the yield of active oxygen forms in neutrophils of healthy donors in vitro at the adhesion of cells on the glass. «Granocyte» induces the clianges of the kinetics of active forms generation at actions of cell metabolism stimulators for different healthy donors. Myeloperoxidase activity of neutrophils is not changed under the influence of granulocyte colony-stimulating factor «Granocyte» in vitro. It was supposed, that granulocyte colony-stimulating factor «Granocyte» modifies activity of the plasma membranes redox-systems, but does not influence on intracellular oxidases.