Biopolymers and cell. 1998; 14 (1): 82 - 86
Interaction of cyanine dyes with nucleic acids. 4. Efficient 5'-fluorescent labelling of oligonucleotides with monomethyne pyrylium cyanine dye, Cyan 39
Sergiy M. Yarmoiuk, Alexander M. Kostenko, Olga S. Kornushyna, Igor Y. Dubey
A new system for fluorescent 5'-end labelling of oligonucleotides with cyanine dyes is proposed. Reaction of primary amine with pyrylium salt was used for the efficient conjugation of monomethyne pyrylium cyanine dye with 5'-aminoalkyl oligonucleotide with simultaneous conversion of the «brightless» pyrylium dye Cyan 39 into «bright» pyridinium one Cyan 40.