Biopolymers and cell. 1997; 13 (5): 419 - 421

Interaction of cyanine dyes with nucleic acids. 3. The of new cyanine dyes Cyan 13 and Cyan 40 for detection of nucleic acids in agarose gel

Sergiy M. Yarmoluk, Igor Ya. Dubey

Detection of double-stranded DNA (dsDNA) and single-strc'nded DNA (ssDNA) and RNA with two new cyanine dyes Cyan 13 and Cyan 40 is reported. Cyan 13 and Cyan. 40 bind to nucleic acids to form a stable fluorescent complexes and can be used for the detection of DMA and RNA samples: separated by gel electrophoresis. Sensitivity of detection is comparable to tfat for ethidium bromide (EtBr), a common nucleic add staining dye.