Biopolymers and cell. 1997; 13 (5): 391 - 396

2',5'-oligo(A)synthetase activity during early metabolism reorientation in regenerating rat liver

M. Obolenskaya, M. Kelve, L. Sazonova

This paper addresses the verification of our hypothesis about the possible participation of 2' ,5'-oligo(A)synthetase — RNAsc L sys¬tem in the metabolic reorientation of regenerating rat liver during the transit from the quiescence to the proliferation induced by partial hepatectcmy (PHE). We analyzed the activity of 2',5'-oligo(A)synthetase, and the dimers/trimers ratios of newly synthe¬sized oligoadenylates. The changes occur in the interval 0.5—3 h after PHE, corresponding to the early period of metabolic re-orientation. They reveal the down-regulation of enzymatic activity and the up-regulation of dimers/trimers ratio in nudeoplasm and up-regulation of enzymatic activity in cytoplasm. The temporal correlation of these changes with those previously obtained i. e. with the retain of newly synthesized RNA in the nucleus and with the reorganization of ribosomes on endoplasmatic reticulum supports our hypothesis though does not yet prove it.