Biopolymers and cell. 1997; 13 (5): 380 - 385

The effect of phosphocreatine OB the distribution of different subunits of translation elongation factor 1 in gently permeabilized human fibroblasts

Boris S. Negrutskii

Highly effective permeabilization/translation system developed recently (Negrutskii et al. (1994) Proc. Nat. Acad, Sci. USA 91, 964—968} was used to study the distribution of FITC-labeled a and S submits of translation elongation factor 1 in human fibroblasts. Somewhat granular fluorescent picture observed for the both proteins distribution during active protein synthesis may be indicative of putative protein synthesis compartments. Exogenous phosphocreatine, an absolutely required agent to support the protein synthesis in permeabilized cells, is shown to influence the EF-la distribution, while not affecting the EF-1S. Most of EF-ia under «low energy» condition is found in nucleus. Thus, the possibility of the functional change in EF-la distribution is demonstrated supporting the Idea about transient character of EF-la involvement into «hea.vy» EF-I complex.