Biopolymers and cell. 1997; 13 (3): 209 - 212

Comparative analysis of histone HI and HI structure and properties

A. F. Protas

The properties of histones HI and HI from rat brain cortex free in solution and in chromatin were studied. Histones HI and HI have the constants for quenching of own fluorescence witit acrylamide 1,4 M and 2,0 M and the binding constants Kias with 1,8-ANS 14,6 MM u 5,5 MM. Histone HI forms high-molecular weight aggregates in solution with NaCl cristals very poorly as well as polymers in chromatin. It is supposed that histone HI function in chromatin is realised on a local site.