Biopolymers and cell. 1997; 13 (1): 18 - 21

The use of polyamines for creation of effective reconstructed protein synthesizing system from rabbit liver

S. S. Palchevskii

A cell-free reconstructed protein-synthesizing system from rabbit liver has been developed. The system contains highly purified homologous components of translation apparatus and is characterized by a sufficient rate of polypeptide synthesis. The use of spermine and spermidine has allowed to optimize the activity of the system at physiological concentration of Mg.