Biopolymers and cell. 1996; 12 (5): 29 - 33

DNA probe construction with the use of silver salt. 3. Dosed modification of two-stranded probe and additional colouring of hybrids

A. V. Shugalii, M. V. Lichina

The different ways of the coloured probe preparation with the use of the metal complex comprising ethanol-amine-formaldehyde chains forming coordination bonds with silver ions have been discussed. The denatu-rated DNA. partially unwind with formaldehyde molecules, and the probe-template duplexes have been modified on the basis of the branching ofoligomer chains already formed. The sensitivity of the detection of 10 % -modified Off A immobilized on nitrocellulose filters has reached dozens ofpicogramms.