Biopolymers and cell. 1996; 12 (3): 77 - 90

The molecular mechanisms of genotoxic action of chlorophose. The in vivo and in vitro investigations

E. L. Levitsky, Yu. I. Gubskiy, R. G. Primak, A. G. Goryushko, A. N. Marchenko

The in vivo (injections into rats) and in vitro (addition to the rat liver chromatin fractions) researches of genotoxic action of phosphororganic pesticide chlorophose perrnitted to reveal some molecular mechanisms of this one. The opposite direction of the changes of some parameters which are characterize the chromatin structure in vivo and in vitro permit to confirm that the poison which is injected to animals probably is modificated to a number of the free radical products, they are in them turn the starting mechanism of the free radical chromatin damage in result in the change of chromatin-bound lipids peroxidation. The developing in result of this the peroxidating chromatin damage is expressed first of all as its DNA and histories structure changes which involves the distortion of the genetic information realization. In this conditions the replicative activity is decreased and the transcriptional one is increased in the transcriptionally active chromatin which involves the increased label incorporation in the proteins of the repressed chromatin fraction. The change of the chromatin functional activity under the chlorophose genotoxic influence is depended on as the modification of the chromatin temp late features as the change of the replication and transcriptional enzymic apparatus.