Biopolymers and cell. 1996; 12 (2): 42 - 46

Genetic transformation of potato (Solanum tuberosum L.) using a binary Agrobacterium tumefaciens vector with patatin promoter class I

P. G. Kovalenko, I. M. Yefimenko, N. V. Schuman, T. V. Medvedeva, K. G. Gazaryan1, A. P. Galkin

Kanamycin resistant plants ofS. tuberosum L. (in vitro-grown) cv. Zarevo were obtained from tlie cocultivated microtubers with A. tumefaciens. A disarmed binary vector systems containing the neomycin phosphotransferase (NPTII) gene as selectable marker and chloramphenicol acetyltransferase (CAT), as a reporter gene, under control of new patatin promoter class I were utilized. In vitro grown minitubers discs were used as sources of explants to produce transgenic plants on selective medium containing 100 fig/1 kanamycin and CAT enzyme activities were detected.