Biopolymers and cell. 1996; 12 (1): 75 - 85

Antiadenovirus and immunostimulating actions of 6-azacylidine :

L. N. Nosach, N. S. Dyachenko, A. S. Shalamay, I. V. Alekseeva, L. Ya. Kushko, 1.1. Ozvinchuk, V. L. Zhovnovataya, S. L Butenko, I. A. Petrovskaya, G. N. Dra.nn.ik

The influence of an original cytidine analogue, 6-azacytidine (6-AC), on the reproduction of a human type I adenovirus in HeLa cell cultures, on a synthesis of nucleic acids aswellasonsome characteristics showing the state of the cell culture monolayer and functional activity of various populations of immunocompetent cells in vitro has been studied. It was established that the 6-AC effectively inhibits the adenovirus reproduction in a wide concentration range, has a high antiviral index (62), and in vitro conditions it possesses the immunomodulating action stimulating the T-lymphocyte proliferation, phagocytic activity and activity of natural killers. Depending on the concentration, the 6-AC exerts a variable action on a synthesis of nucleic acids, particularly on DNA in the non-infected and infected cells: its high concentrations, which completely or almost completely inhibit the adenovirus reproduction, decrease nucleic acids synthesis; less concentrations stimulate it suppressing adenovirus reproduction for 30 % or less. The 6-AC concentrations differently inhibiting the adenovirus reproduction did not exert the cytodestructive effect on the monolayer cell culture, butthey inhibit its mitotic and growth activities.