Biopolymers and cell. 1996; 12 (1): 64 - 68

Mapping of Bacillus subtilis riboflavin operon fragment coding for 3 ,4-dihydroxy-2-butanone-4-phosphate synthase

N.I. Boretska, O. Ye. Liauta-Teglivets, A. Y. Voronovsky, J. R. Boretsky, G. M. Shavlovsky

Complementation analysis of Bacillus subtilis riboflavin operon was carried out by means of Escherichia coli riboflavin deficient strains and recombinant plasmids. It was found that ORF-3 ofB. subtilis riboflavin operon encodes for a bifunctional enzyme. It catalyses formation of both 2, 5-diamino-4-hydroxy-6-ribosylamino-pyrimidine-5'-phosphate from GTP and 3, 4-dihydroxy-2-butanone-4-phosphate from ribuhse-5-phosphate.