Biopolymers and cell. 1995; 11 (6): 96-103

Organ-specific gene expression in transgenic potato: the cloning a new promoter of a class I patatin gene

YEFIMENKO I. M., MEDVEDEVA T. V., KOVALENKO P. G., GAZARYAN K. G., GALKIN A. P.

Using synthetic oligonucleotide probes homologous to conservative AT-rich motif of patatin genes class I of two different clones were isolated from a potato genomic library. One of two different genomic clones named ?122 was subcloned and analysis 5'-region sequences. Using the chloramphenicol acetyltransferase (CAT) gene as a reporter it has been shown that a 1.8 kb promoter fragment of the class I patatin gene PAT 122 provides all the information necessary for both tuber-specific and sucrose-induced expression in leaves in transgenic potato plants.