Biopolymers and cell. 1995; 11 (3-4): 96 - 103

STUDDING OF ROLE SITE PLASMIN FOR RELATION WITH LYSIiNE AND ARGININE ON EARLY STAGES FIBRIN(OGEN) HYDROLYSIS

E. N. Zolotareva

The effect of 6-aminohexane acid (6-AHA) and L-arginine on the rates of early stages of fibrinogen hydrolysis by Lys77-plasmin, Val^-plasmin and Lys53o-plasmin were stu¬dded. It was shown that 6-AHA on the second reaction stage (hydrolysis of X) in the concentrations saturating the site, of plasmin heavy chain had a strong inhibiting influ¬ence even more stronger than a the first stage (hydrolysis of fibrinogen). Effect of arginine and 6-AHA on the first stage hydrolysis was identical. Arginine effect on the second stage failed to be observed while its concentration were effecting kringle 1 and 5. It was suggested the enzyme interaction with substrate took place on the first stage of hydrolysis under kringles 5 and 1 patrkipation on second stage — under kringle 4 participation.