Biopolymers and cell. 1994. Volume 10. № 6. 88 - 91

KALACHNYUK L. G.

Individual Isoacceptor Tyrosine tRNAs From Bovine Liver. Isolation and Characteristic

Summary

    Combination of two chromatographies of BD-cellulose in the presence and in the absence of Mg2+ was applied for high yield isolation of individual isoacceptor tyrosine tRNAs from bovine liver. The final purifacation of tyrosine tRNA was achieved by gel electrophoresis on a 7% gel at 7 M urea. The nucleotide sequence of tRNATyr1 from bovine liver was determined to be identic to primaty structure of tyrosine tRNA Q*wA from bovine liver (13) ( minor bases was not determined).