Biopolymers and cell. 1994. Volume 10. № 6. 45 - 51

N. V. Zheltovsky, S. A. Samoilenko, I. N. Kolomiets, I. V. Kondratyuk, A. V. Stepanyugin

INTERACTIONS OF METHYL AND GLYCOSYL DERIVATIVES OF PYRIMIDINE NUCLEOTIDE BASES WITH AMINO ACID CARBOXYLIC GROUP

Summary

    By UV, IR spectroscopies and PMR interactions of a number of methyl and glycosyl. derivatives of pyrimidine bases with amino acid carboxylic group in DMSO were studied as a model of probable point contacts in real nucleoproteid complexes. It was found that like the unsubstituted base 1-methylcytosine, 5-methylcytosine, 1,5-dimethylcytosine, 'cytidine, deoxycytidine, 5-methyldeoxycytidine form complexes with a neutral carboxylic group, and 3-methylcytosine and isocytosine interact with deprotonated carboxylic group (carboxylate ion). Like free thymine and uracil, their derivatives 1-methylthymine, 1-methyluracil, 3-methyluracil, thymidine, uridine, 5-methyluridine, deoxyuridine interact only with carboxylate ion. Analysis of the results obtained and the data of previous works points out the ability of amino acid carboxylic group to differentiate between cytosine and thymine (uracil), and between their nucleosides and derivatives as well.