Biopolymers and cell. 1994. Volume 10. № 3-4. 89 - 92

A. I. Zayas, I. N. Stekhin, N. V. Putilina, B. A. Levenko

CREATION OF THE EXPRESSING CONSTRUCTIONS WITH BAR GENE FOR PLANT TRANSFORMATION

Summary

    Phosphinothrioin (PPT) is a potent inhibitor of glutamine synthetase in bacteria and plants and is used as a non-selective herbicide. The bar gene which confers resistance ire Streptomyces hygroscopicus to bialaphos, a tripeptide containing PPT, was cloned. The bar gene was placed under control of the 35S promoter of the cauliflower mosaic virus for transferring to plant cells using agrobacterium—mediated transformation.