Biopolymers and cell. 1994. Volume 10. № 3-4. 84 - 88

A. I. Zayas, I. N. Stekhin, N. V. Putilina, B. A. Levenko

CLONING OF THE HERBICIDE BIALAPHOS RESISTANCE GENE

Summary

    A gene which confers resistance to the herbicide bialaphos (bar) has been cloned from Streptomyces hygroscopicus genomic DNA. The bar gene was cloned in E. coli using the vector pUCfff. The recombinant clones, resistant to bialaphos in concentration 0,005 mg/ml, were obtained. Plasmid DNA isolated from resistant clones in each case contained the same 1,7-kb PstI fragment. Restriction mapping of this fragment has been made.