Biopolymers and cell. 1994. Volume 10. № 3-4. 79 - 83

E. I. Cherepenko, O. I. Karpenko, S. S. Maluta

GENETIC MECHANISMS OF ESCHERICHIA COLI CELL RESISTANCE TO AMINO ACID ANTIMETABOLITES. 1. SEARCH FOR GENES OTHER THAN GLYPHOSATE TARGET GENE

Summary

    Conjugation donor Hfr C prototrophic cells were treated with NNG and glyphosate resistant cells were selected. Out of 50 mutants obtained 36 transferred the trait of glyphosate resistance to recipient cells showing that only a single gene was mutated. No changes in glyphosate penitration into mutant cells studied were shown. No changes in Coti/2 of mutant DNA were found which could be if pronounced amplification of aroA locus occurred. Mutants obtained were shown to be localized at 4 located far from each other and from aroA gene.