Biopolymers and cell. 1994. Volume 10. № 3-4. 75 - 78

E. I. Cherepenko

ColEl PLASMIDS CAN PREVENT THERMOINACTIVATION OF PHENYLALANYL-tRNA SYNTHETASE IN ESCHERICHIA COLI

Summary

    Because RNA I controlling ColEl plasmid replication is tRNA-like structure and has consensus and homological sequences of tRNAphe we studied the ability of this molecule to complement thermolabile phenylalanyl-tRNA synthetase in vivo just like tRNAphe can do. We showed that cells containing this enzyme and transformed with pUC19 and pBR322 plasmids could grow at 42 °C when grown overnight with aeration in diluted meat broth incubating all transformants at a once which resulted from transformation procedure being diluted by a factor of 50.