Biopolymers and cell. 1994. Volume 10. 2. 24 -

 

H. V. Turkovskaya, V. F. Shalak, K. V. Semenikhin, B. S. Negrutskii

 

FAST PROCEDURE FOR ISOLATION OF PHENYLALANYL-tRNA SYNTHETASE FROM RABBIT LIVER

 

Summary

 

    Simple and fast method for isolation of phenylalanyl-tRNA synthetase from rabbit liver is proposed. Three-step procedure includes polyethylene glycol precipitation of post-rnitochondrial supernatant with subsequent DEAE-cellulose and phosphocellulose chromatographies.