Biopolymers and cell. 1994. Volume 10. № 2. 17 - 27

N. A. Kozyrovska, M. F. Alexeyev, G. L. Kovtunovich, N. V. Gun'kovska, V. A. Kordyum

BIOLUMINESCENCE-BASED DETECTION OF KLEBSIELLA OXYTOCA VN13 IN THE ENVIRONMENT

Summary

    The continuous expression of the Photobacterium leiognathi 54D10 lux genes coding for the bioluminescence was obtained in Klebsiella oxytoca VN13. Chromosomally and plasmid-encoded bioluminescence of strains constructed was used to monitor their survival in the barley rhizosphere during a limited field introduction in parallel with the parental strain. Lux+ Derivatives of K. oxytoca VN13, carrying the bioluminescence reporter plasmids survived on roots during the whole vegetative period of the plant and were not isolated from soil.