Biopolymers and cell. 1993. Volume 9. № 6. 13 - 21

LEVITSKY E. L., GUBSKY Yu. I., CHABANNY V. N., VOLKOV G. L., NOVIKOVA S. N.

Biochemical Characteristics of the Rat Liver Transcriptionally Active and Repressed Chromatin

Summary

    The intact rat liver trancriptionally active and repressed chromatin fractions have been characterized. The transcriptionally activity differences between the fractions may be conditioned by distinctions in the chromatin protein component, that is increased RNA-polymerases concentration in active chromatin. Differences between in vivo and in vitro DNA synthesis, thermodenaturation process and susceptibility to endo- and exogenous DNA-ases are insignificant and so it may be thought the DNA structure similarity in active and repressed chromatin. Moreover it may be thought that differences between phospholipid and fatty acids fractions compositions may be a cause of the chromatin realization of its genetic activity.