Biopolymers and cell. 1993. Volume 9. № 2. 19 - 23

ADARICHEV V. A., DYMSHITS G. M., MISHINA E. S., SALGANIK R. I., SOCHANENKOVA T. L.

Nonradioactive Hybridization Test of Plasmodium falciparum Infection of Humans

Summary

    Nonradioactive molecular hybridization test on Plasmodium falciparum infection in human peripheral blood is described. The DNA immobilization both on nylon and nitrocellulose membranes is carry out by UV irradiation. Nonradioactive biotinilated probe is prepared according to original method based on chemical modification of doublest-randed DNA. The correlation coefficient between microscopic examination data and hybri¬dization data is 0.96 for infection density more then 25 parasites by 1000 leukocytes.