Biopolymers and cell. 1993. Volume 9. № 1. 39 - 44

TITOK T G., KOSTETSKY I. E., TCHAIKOVSKAYA T. L., VARZANOVA I. S., KOCHUBEY T. P., LUKASH L. L., KORDIUM V. A.

Transplantation of Human Preproinsulin Gene Into the Insuline Dependent Diabetic and Normal Rats

Summary

    The encapsulated into liposomes recombinant plasmid DNAs carrying the human preproinsulin gene sequences (pAINS and GINS) were injected into the liver and the intraperitoneum of streptozotocin and autoimmune insulin-dependent diabete rats. The injection of plasmids decreased glucose concentration in serum of streptozotocin diabetic rats. Maximal effect was detected in 8—10 hr after injection and glucose concentration returned in 24 h to initial levels. The injected diabetic BB rats not display a similar response. There was noticeable immunoreactive product concentration variation in raf blood serum after injection. Possible endocrine and immunological mechanisms of this.effect are discussed.