Biopolymers and cell. 1992. Volume 8. 6. 55 - 57

 

ALEXEYEV M. F.

 

Cloning in the laclq of pTTQ plasmids: «Reverse» Colour Reaction

 

Summary

 

    The method for cloning Mlul-, Bell-, A pal-, BssHI- and £co7?l/-DNA fragments in the laclq gene of pTTQ plasmids is described. Recombinant clones are se¬lected as coloured in blue colour colonies on plates containing ampicillin and X-gal.