Biopolymers and cell. 1992. Volume 8. № 6. 26 - 30

AVERINA T. I., DRAGOVOZ V. A., RODNIN N. V., SIDORIK L. L.

The Investigation of SerRS distribution in Tissues of Higher Eukaryotes With Immunochemical Methods

Summary

    The immunochemical approach to the study of enzyme distribution in eukaryotes (in different organs and tissues) gives a possibility to determine not only quantity of the protein but the molecular weight of the subunit without purification. The study of aminoacyl-tRNA synthetases distribution in different organs and tissues, comparison of the protein quantity determined by immunochemical methods with the enzyme activity is important for the study of so-called uncanonic functions of this class enzymes, We have studied the distribution of Ser RS in organs and tissues of the different species of Vertebrates by immunochemical methods. It is shown that the highest Ser RS level is observed in the liver (from fish to humans). Ser RS partially exists in the form of high-molecular complex that is stable in the lysates even under denaturating conditions.