Biopolymers and cell. 1992. Volume 8. № 4. 56 - 58

DANKO I. M., PRIMA V. I.

Replication of Oncogene c-myc in Ehrlich Ascitic Cells

Summary

    Sedimentational fractionation of asynchronic Ehrlich ascitic cells po¬pulation was carried out according to cell cycle phases. Separation of tumor cells sus¬pension was carried out under 1 g in 0—0,3 M sucrose concentration gradient. Cells, pituated in S phase, were selected to 3 fractions in early, midle and late S phase respec¬tively. It was studied temporal order replications of synchronized Ehrlich ascitic cells. It was shown that the replication of oncogene c-myc beginning in early S phase.