Biopolymers and cell. 1992; 8 (2): 44 - 49

PANIN L. E., POLYAKOV L. M., USYNIN I. Ph., KUZMENKO A. P., POTERYAEVA O. N.

Immunochemical Identification of Apoprotein A-I in the Chromatin of Rat Hepatocyte Nuclei

Summary

    Two proteins of 28000 and 14000 immunologically identifical to apopro-tein A-I were found in the chromatin of rat hepatocyte nuclei of using immunoblotting and enzyme-linked immunosorbent assay. The 28 000 protein was present in the trans-criptionally active chromatin and in the nuclear matrix. The 14000 protein was present in the repressed chromatin. The molecular weights of these protein determinated by .SDS-PAGE, and immunochemical identity allow us to propose, that one of this proteins is apoprotein A-I, and the other one is probably a product of limited proteolysis of apo-protein A-I.