Biopolymers and cell. 1992. 8 (1): 101 - 107

SARA SANA, MOZURAITIS R. J., KHARCHENKO O. V., IVANOV L. L, TURKOVSKAYA G. V., MARTINKUS Z. P., KOVALENKO M. I., EL'SKAYA A. V.

Interaction of Eukaryotic Aminoacyl-tRNA Synthetases With Ribosomes

Summary

    The activities of eukaryotic aminoacyl-tRNA synthetases associated with polyribosomes have been determined under the different levels of protein biosynthesis. A decrease of some aminoacyl-tRNA synthetase activities in the fraction of polyriboso¬mes as well as in the fractions of free and membrane-bound ribosomes has been obser¬ved during experimental myocardial ischemia, which results in the reduce of the trans-lational level in cell-free system. The activity of leucyl-tRNA synthetase in the fraction of cow mammary gland polyribosome has been increase under lactation. The stimulating effect of 40S and COS ribosomal subunits and SOS ribosomes on the activity of rabbit li¬ver leucyl-tRNA synthetase as well as on the positive cooperativity of tRNA-binding si¬tes has been revealed.