Biopolymers and cell. 1992. 8 (1): 89 - 100

SIVOLOB A. V., KHRAPUNOV S. N.

Fluorescence Spectroscopy in the Investigations of Protein-Nucleic Acids Interactions in Chromatin

Summary

    The brief review dealt with the investigations of chromatin molecular or¬ganization by the fluorescent methods. The results of chromatin and nucleosome studies, which have been obtained by using of proteins and DNA labelling by fluorescent dyes, as well as the data of intrinsic protein fluorescence obtained mainly by the authors, are described. The advantages of the different fluorescent approaches, which allow to obtain a unique information about protein-nucleic acids complexes structure and dynamics, are demonstrated and discussed.