Biopolymers and cell. 1992. 8 (1): 62 - 72

RADCHENKO E. D., REVA I.D., PLOKHOTNICHENKO A. M., STEPANIAN S G SHEINA G. G., BLAGOI Yu. P.

Investigation of the Glycine Molecular Structure by the Matrix IR-Spectroscopy

Summary

    Dependences of the observed hyperchromism on DNA concentrations for DNA with different bases contents in the water were studied by UV-spectroscopy. It has been shown that DNA protonation begins with AT-pairs in the water and at low ionic strengths. The dependences of protonation constants values of AT-pair Ki on counterion •concentration were obtained. It was carried out the comparison K\ with protonation constant of GC-pair Aa at different ionic strengths. Experimental data earlier obtained on the basis of proposed mechanism of protonation are discussed.