Biopolymers and cell. 1991. Volume 7. № 6. 5 - 15

VESELKOV A. N., DAVIES D. B., DJIMANT L. N., PARKES H. G.

Investigation of Interaction of Proflavine With Deoxytetraribonucleoside Triphosphate 5'-d(ApCpGpT) by the Method of 1H-NMR spectroscopy

Summary

    Complex formation between acridine dye proflavine and self-complementary deoxytetra-ribonucleoside triphosphate 5'-d(ApCpGpT) in water-salt solution has been studied by the method of one-dimensional and two-dimensional 'H-NMR spectroscopy (500 MHz). Two-dimensional homonuclear 'H-NMR spectroscopy (2D-COSY and 2D-NOESY) was used for complete assignments of proton signals of molecules in solution and for qualitative analysis of the nature of interactions of proflavine with tetranucleotide. Concentration de¬pendences of proton chemical shifts of the molecules have been measured at 293 K. Ana¬lysis of relative content of different types of complexes has been made and special featu¬res of dynamic equilibrium have been revealed as a function of correlation of dye and tetranucleotide concentrations. Based on the obtained results a conclusion has been made about preferential intercalation of proflavine to CG-site of tetranucleotide in duplex form. The most favourable structure of 1 : 2 complex of dye with tetranucleotide has been const¬ructed using calculated values of induced chemical shifts of proflavine protons and 2D-NOESY spectra.