Biopolymers and cell. 1991. Volume 7. № 5. 15 - 23

VESELKOV A. N., DJIMANT L. N., DAVIES D., PARKES H., SHIPP D.

ID- and 2D-1H NMR Investigation of Self-Association of Deoxytetraribonucleoside Triphosphates of Different Base Sequence in Aqueous Solution

Summary

    Equilibrium behaviour of self-complementary deoxytetranucleoside triphosphates 5'-d(GpCpGpC), 5'-d(CpGpCpG), S'-d(ApGpCpT) and 5'-d(ApCpGpT) in aqueous solu¬tion have been studied by the method of PMR spectroscopy (500 MHz). Assignments of the tetranucleotides protons have been obtained from both two-dimensional homo-nuclear COSY and NOESY experiments. Concentration and temperature dependences of proton chemical shifts have been investigated. Equilibrium association constants and thermodynamical parameters of molecules self-association have been calculated using theoretical models and regression equations based on experimental results.