Biopolymers and cell. 1991. Volume 7. № 4. 72 - 80

ZUBKO M. K., ZUBKO E. I., KAPRANOV Ph. V.

Induction of Chlorophyll Deficient Tobacco Mutants as Markers for Cell Engineering

Summary

    Chlorophyll deficient Nicotiana tabacum mutants with the different phenotypes were induced using gamma-irradiation of seeds and haploid protoplasts. Cytoplasmic or geno-mic type of chlorophyll-deficiency inheritance has been shown for part of them. Experiments on protoplast fusion for N. tabacum, Atropa belladonna, Hyoscyamus niger and Scopolia carniolica have conformed the possibility to involve albino mutants in projects on close related and remout somatic hybridization. Simple and effective procedure for fusion of chlorophyll-less protoplasts is proposed.