Biopolymers and cell. 1991. Volume 7. № 3. 13 - 20

ENGELHARDT P., AKHMEDOV A. T., LANZOV V. A.

RecA-Like Proteins in Nuclei of Higher Eukaryotes: Detection of Pachytene Chromosome Cores in Isolated Unfixed Bovine Meiotic Nuclei with Antibodies to RecA Protein of Escherichia coli

Summary

    Polyvalent antibodies to RecA protein of E. coli (anti-RecA) interect specifically with 40-and 90-kDa polypeptides from pachylene nuclei of bull testes. As it was shown by the indirect immunofluorescence technique, using of the anti-RecA in unfixed isolated nuclei at different mciotic stags revealed the uniform staining of fin ineiotic chromosome cores with bright staining dots (sometimes of telomeric localization) surrounded by DNA counterstaing with propidium iodide. This picture suggests binding of anii-RecA with components of synaptonemal complexes that is expressed more brightly in the recombination nodules during chiasma formation. The staining by anti-RecA was more feeble and difiusible in premeiotic cells. In spermatozoa antigenes are revealed in the dorsal or acrosome-like material of their heads.