Biopolymers and cell. 1991. Volume 7. № 2. 80 - 86

GORBULEV V. G., CHERNOV B. K., RUBTSOV P. M.

Cloning of cDNA Encoding Bovine Follicle-Stimulating Hormone

Summary

    The XgtlO library of bovine pituitary cDNA was screened and the clones with cDNA encoding a subunit of glycoprotein hormones were isolated. The sequencing of the inserts from two clones revealed that they containe 3'-terminal nucleotide of mRNA followed by the prolonged (about 300 bp) but different stretch. The clones carrying the cDNA for f, subunit of follicle-stimulating hormone (FSH) were isolated from the enriched plasmid library of bovine pituitary cDNA. The sequence of cDNA for |3 subunit of FSH was established. The sequence results proved that we have cDNAs encoding the full-length both a and f5 subunits of bovine FSH.