Biopolymers and cell. 1991. Volume 7. № 1. 101

T. V. Sorochynskaja, S. I. Chernykh

Study of p-lactamase tem1 synthesis in various escherichia coli strains infected by the phage k bla with amber-mutations in different regulatory genes

Summary

    The search of optimal conditions for phage-dependent supersynthesis of (?-lactamase was undertaken. For this purpose the recombinant phage with alternatively orientated p-lactamase (bla) structure gene were obtained. It was stated, that phage XblaN-N~, XblaQ-R- derivates are obtained. The p-lactamase producing strain was selected. The maximal recovery of p-lactamase was obtained while Su° E. coll W3101 strain was infected by X-phage bla Q-R-.