Biopolymers and cell. 1991. Volume 7. № 1. 63 - 70

GNATENKO D. V., KORNELYUK A. I., KUROCHKIN I. V., MATSUKA G H

High Molecular Weight Complex of Tyrosyl-tRNA Synthetase from Bovine Liver

Summary

    The tyrosyl-tRNA synthetase in postribosomal bovine liver supernatant is included as a compound of the high molecular weight complex with Mr>106 Da. Dissociation of tyro¬syl-tRNA synthetase from this complex due to proteolysis proceeds through the intermediate labile complex with Mr~240 kDa. The thermostability of tyrosyl-tRNA synthetase at this intermediate labile complex is much higher in comparison with free enzyme. The labile intermediate complex of bovine liver tyrosyl-tRNA synthetase possesses the nonspecific affinity for high molecular weight ribosomal RNA.