Biopolymers and cell. 1990. Volume 6. № 5. 86 - 90

E. L. Babiychuk, S. G. Kushnir, Yu. Yu. Gleba

Fertile Intertribal Symmetrical Somatic Hybrids in the Solanaceae

Summary

    Usage of the genetically homogeneous, euploid mesophyll protoplasts and strict geneti-cal selection is supposed as particularly important rule for production of the normal remote somatic hybrids. As a result the performed experiments, morphologically normal hybrids Nicotiana tabacum+Atropa belladonna, Nicotiana tabacum+Physochlalne officinalis possessing symmetrical quantities of the nuclear genetic material from both parents are obtained, that follows from the carried out cytogenetical and biochemical analyses. Sexual progeny was obtained after pollination of N. tabacum+A. belladonna hybrids with tobacco pollen. Fl plants possess 20-30 chromosomes of A. belladonna (2rc=72) and 48 chromosomes of N. tabacum (2«=48). Some important problems of genetics, physiology, developmental biology and genetical control of the secondary metabolite boisynthesis could be studied using described hybrids and their progeny. As is generally known described symmetrical somatic hybrids are the first fertile plants combining full nuclear genomes of the plants from different tribes.