Biopolymers and cell. 1990. Volume 6. № 5. 72 - 77

L. L. Sidorik, V. I. Popenko, N. E. Cherny, M. A. Tukalo, S. F. Beresten, G. Kh. Matsuka

IMMUNOELECTRON-MICROSCOPIC STUDY OF SERYL-tRNA-SYNTHETASE LOCALIZATION IN CELLS OF HIGHER EUCARYOTES

Summary

    Localization of seryl-tRNA-synthetase (SerRS) was studied on ultrathin (UT) section of bovine kidney cells fixed with glutaraldehyde and embedded in «Lowycril K4M» resin at —35 °C. The UT sections were treated with the polyclonal affined-purified antibodies against SerRS and the complexes of protein A with colloidal gold 20 nm in size. In the cells the cytoplasm was the most intensely labelled. A great amount of SerRS was found in the cell nuclei: the label was concentrated over the diffuse chromatin localiza¬tion regions, a minimal binding being observed over compact chromatin. The presence of SerRS in the eucaryotic cell nucleus may evidence for the possible regulatory role of SerRS in eucaryotes.