Biopolymers and cell. 1990. Volume 6. № 4. 92 - 96

S. P. Kovalenko, N. P. Mertvetsov

EXPRESSION IN ESCHERICHIA COLI OF THE HUMAN ANGIOGENIN GENE TRANSLATIONALLY FUSED WITH THE N-TERMINAL FRAGMENT OF 0-GALA-CTOSIDASE

Summary

    The phage M13mp8 earring a DNA fragment coding for fusion protein angiogenin-a-peptide of (3-galactosidase was obtained by oligonucleotide directed mutagenesis techni¬que from the M13mp8 phage with a cloned synthetic human angiogenin gene. The syn¬thesis of peptide with, cc-donor activity in the phage Ml-1-infected cells was demonstra¬ted. The amount of the fusion protein was estimated at the different stages of E. coll cell growth after the phage infection.