Biopolymers and cell. 1990. Volume 6. № 2. 100 - 104, 113
L. M. Rumer, V. A. Livshlts
PLASMID TRANSFORMATION OF STREPTOCOCCUS LACTIS SPHAEROPLASTS.
Summary
The optimal conditions for production of transformable spheroplasts of Streptococcus lactis using lysozyme were determined. It has been established that viability of Strepto¬coccus lactis cells fell rapidly when concentration of lysozyme or duration of lysozyme treatment increased. The successful transformation of Streptococcus lactis spheroplasts by two heterolo-gous plasmids pRT84 and pLF2 was performed. It was found that expression of Bacillus amylollquefaciens gene coding for amylase cloned in pRT84 took place in Streptococcus lactis cells.