Biopolymers and cell. 1990. Volume 6. № 2. 91 - 100
I. V. Kroupskaya, A. N. Zhyvoloup, E. B. Paton
CONSTRUCTION OF HYBRID LACZ GENES TO STUDY THE E. COLI RPLJL OPEROX GENES EXPRESSION MECHANISMS
Summary
Using the pNM481 plasmid vector 5'-terminal fragments of rplJ and rplL genes were fused in-frame to gene lacZ. Hybrid p-alactosidases activity, encoded by genes rplJ'— lacZ and rplL'-lacZ was compared. In contrast to rplL'-lacZ, extension of rplJ fused portion resulted in the decrease of the p-gal activity level. Different efficiency of translations seems to be the most probable mechanism, providing the excess synthesis of r-protein L7/L12 in vivo.