Biopolymers and cell. 1990. Volume 6. № 1. 96 - 99
G. D. Telegeev, I. V. Zozulla, S. S. Maliuta
EXCISION OF HeLa QE'NOMIC REPEATED SEQUENCES AS A POSSIBLE WAY TO PRODUCE MINICIRCULAR DNA
Summary
Using cloned minicircular DNA(mcDNA) of HeLa cells a number of its genomic copies has been studied. It is found that both probes have chromosome homology and are presented at least by 800-900 copies. The cycloheximide treatment and «ageing» in vitro results in a 0.2-2-fold decrease of its genomic copies with simultaneous increase in Cytoplasmic fraction. It is explained by means of recombination and excision of repeats in the circular form.