Biopolymers and cell. 1989. Volume 5. № 6. 101 - 104

I. S. Groisman, A. P. Potapov

THE EFFECT OF ESCHERICHIA COLI RIBOSOMAL PROTEIN S12 MUTATIONS ON THE EFFICIENCY OF DEOXYRIBONUCLEOTIDE MATRIX POLY(dT) TRANSLATION

Summary

    The efficiency of poly(dT) translation has been studied in cell-free systems from wild-type E. coli and streptomycin-resistant mutants with altered ribosome protein S12. The data show that there is a positive correlation between poly(U) misreading and efficiency of poly(dT) translation. Mutant ribosomes translate poly (U)-template more accurately than ribosomes from wild-type bacteria and they are less efficient in translation of poly(dT) as well. The ribosome seems to select codon-anticodon pair as a whole unit. The data are in good agreement with hypothesis of stereospecific stabilization of codon-anticodon complex by the ribosome.